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| Registros recuperados: 31 | |
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| Pires,Prhiscylla Sadanã; Ecco,Roselene; Silva,Rodrigo Otávio Silveira; Araújo,Marina Rios de; Salvarani,Felipe Masiero; Heneine,Luíz Guilherme Dias; Oliveira Júnior,Carlos Augusto de; Lobato,Francisco Carlos Faria. |
| ABSTRACT: A standardized immunochemistry method for the diagnosis of clostridial myonecrosis was applied to 38 formalized tissue samples from ruminants with clinical and post mortem history suggestive of blackleg or gas gangrene. The diagnosis of clostridial myonecrosis was confirmed in 37 out of 38 (97.4%) samples tested. Clostridium chauvoei and Clostridium perfringens type A were the most common agents found alone, being detected in ten (26.3%) and six (15.8%) samples, respectively. The other cases showed an association of two or three clostridia, with C. perfringens type A detected in 11 (29%) cases. Based on the findings of the present study, polyvalent vaccines against clostridial infections of animals incorporating C. perfringens would be more... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gas gangrene; Blackleg; Malignant edema; Cattle; Sheep; Immunohistochemistry. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000100452 |
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| Ramos,Carolina Pantuzza; Xavier,Rafael Gariglio Clark; Leal,Carlos Augusto Gomes; Facury Filho,Elias Jorge; Carvalho,Antonio Ultimo de; Viegas,Flávia Mello; Pires,Isadora Honorato; Lopes,Emily Oliveira; Lobato,Francisco Carlos Faria; Silva,Rodrigo Otávio Silveira. |
| ABSTRACT: The present study aimed to describe and characterize, for the first time, two outbreaks of salmonellosis caused by Salmonella Ndolo in foals and calves in Brazil and compare the isolated strains with S. Ndolo previously identified in asymptomatic reptiles. The affected calves and foals presented fever, lethargy, and profuse diarrhea. Isolated strains were subjected to antimicrobial susceptibility testing, characterized according to virulence genes, and fingerprinted by ERIC-PCR. Salmonella Ndolo was identified in fecal samples from two foals and four calves. One isolate from a calf was resistant to amoxicillin/clavulanic acid, trimethoprim/sulfamethoxazole, and florfenicol. Strains from two other calves were resistant to oxytetracycline. All... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonellosis; Zoonoses; Antimicrobial resistance; ERIC-PCR; Salmonella Ndolo.. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001200452 |
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| Silva,Rodrigo Otávio Silveira; Oliveira Junior,Carlos Augusto; Diniz,Amanda Nádia; Alves,Guilherme Guerra; Guedes,Roberto Maurício Carvalho; Vilela,Eduardo Garcia; Lobato,Francisco Carlos Faria. |
| The objective of this study was to evaluate antimicrobial susceptibility in Clostridium difficile strains isolated from animals and humans in Brazil. The 54 C. difficile strains used were isolated from stool samples from piglets (n=16), dogs (n=13), humans (n=13), foals (n=8) calves (n=2), an ocelot (n=1) and a maned wolf (n=1). Antimicrobial susceptibility was determined using the serial plate agar dilution method for penicillin, florfenicol, oxytetracycline, erythromycin, vancomycin, metronidazole and tylosin. The C. difficile strains assessed were susceptible to metronidazole and vancomycin. Florfenicol resistance was rarely observed; 52 (96.4%) strains were sensitive to this antimicrobial. Five (9.3%), five (9.3%), 14 (25.9%) and 20 (37.0%) strains... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nosocomial diarrhea; Pseudomembranous colitis; Resistance. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000500013 |
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| Lobato,Francisco Carlos Faria; Salvarani,Felipe Masiero; Silva,Rodrigo Otávio Silveira; Souza,Andréa Márcia de; Lima,Catarina Guimarães Rocha Dourado; Pires,Prhiscylla Sadanã; Assis,Ronnie Antunes de; Azevedo,Edisio Oliveira de. |
| Botulismo é uma intoxicação causada pela ingestão das toxinas produzidas pelo Clostridium botulinum, que acomete mamíferos e aves. Neste trabalho é descrito um surto de botulismo em ruminantes, ocorrido em duas propriedades localizadas no município de Patos, no Estado da Paraíba, Brasil. Em uma das propriedades, de um total de 88 bovinos, 85 (96,6%) vieram a óbito. Na segunda, morreram 145 ovinos (96,7%), 233 caprinos (57,8%) e 30 bovinos (96,8%). Os animais acometidos apresentavam paralisia progressiva, dificuldade de locomoção, sialorréia e dispnéia. A morte ocorreu entre 24 e 48 horas após o início dos sinais, por parada cardio-respiratória. Nenhuma alteração significativa foi observada no exame post-mortem. O diagnóstico de botulismo foi confirmado... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Botulismo; Clostridium botulinum; Cama-de-frango; Ruminantes. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000400046 |
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| Lobato,Francisco Carlos Faria; Salvarani,Felipe Masiero; Silva,Rodrigo Otávio Silveira; Martins,Nelson Rodrigo Souza; Souza,Andréa Márcia de; Carvalho Filho,Maurício Baltazar de; Nascimento,Ricardo Aurélio Pinto; Assis,Ronnie Antunes de. |
| Botulismo é uma intoxicação causada pela ingestão das toxinas produzidas pelo Clostridium botulinum, que acomete mamíferos e aves, caracterizando-se por um quadro de paralisia flácida. Neste trabalho, é descrito um caso de botulismo em ganso, ocorrido no município de Santa Luzia, região metropolitana de Belo Horizonte, no Estado de Minas Gerais. Ao exame clínico, o animal apresentava-se com um quadro de paralisia flácida dos músculos do pescoço, das pernas e asas, além de apresentar ainda desprendimento de penas. A necropsia não revelou lesões significativas. Foi colhido o soro do animal e submetido ao teste de soroneutralização em camundongo, que identificou a toxina de C. botulinum tipo C. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Botulismo tipo C; Clostridium botulinum; Ganso; Aves. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000400047 |
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| Lobato,Francisco Carlos Faria; Salvarani,Felipe Masiero; Silva,Rodrigo Otávio Silveira; Assis,Ronnie Antunes de; Lago,Luis Alberto do; Carvalho Filho,Maurício Baltazar de; Martins,Nelson Rodrigo da Silva. |
| O botulismo é uma intoxicação causada pela ingestão das toxinas produzidas pelo Clostridium botulinum, que acomete mamíferos e aves e é caracterizado por um quadro de paralisia flácida. Neste trabalho, é descrito um surto de botulismo em perus, ocorrido no município de Santa Luzia, região metropolitana de Belo Horizonte, Minas Gerais. Os animais apresentavam incoordenação motora, paralisia flácida das patas, asas e pescoço. Em um intervalo de 24 horas, todos os 29 animais do plantel vieram a óbito. Na necropsia, observou-se a presença de larvas de mosca no inglúvio. Nos soros coletados, foi identificada a toxina botulínica tipo C pelo teste de soroneutralização em camundongos. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toxina botulínica tipo C; Clostridium botulinum; Perus; Aves. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000100047 |
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| Silva,Rodrigo Otávio Silveira; Oliveira Junior,Carlos Augusto; Guedes,Roberto Maurício Carvalho; Lobato,Francisco Carlos Faria. |
| Clostridium perfringens is a gram-positive, anaerobic, fermentative, spore-forming bacillus that may be found in the environment but is more commonly found as part of the microbiota of humans and animals. The bacterium is considered a common enteric pathogen; however, the pathogenesis and predisposing factors of the disease can differ among species. Therefore, specific studies are necessary for understanding the role of this pathogen, how to diagnose it and which control measures are applicable. The aim of this paper is to review the current knowledge regarding C. perfringens infections in pigs, horses and broiler chickens |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diarrhea; Enteritis; Equine; Swine; Poultry. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000601027 |
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| Silva,Rodrigo Otávio Silveira; Salvarani,Felipe Masiero; Cruz Júnior,Eduardo Coulaud da Costa; Pires,Prhiscylla Sadanã; Santos,Renata Lara Resende; Assis,Ronnie Antunes de; Guedes,Roberto Maurício de Carvalho; Lobato,Francisco Carlos Faria. |
| Clostridium difficile has emerged as a major cause of neonatal colitis in piglets, displacing classic bacterial pathogens. However, there is no information regarding the distribution of this microorganism in pig farms in Brazil. In the present study, the presence of toxins A/B and of C. difficile strains in stool samples from 60 diarrheic or non-diarrheic newborn piglets (one to seven days old), from 15 different farms, was studied. The presence of toxins A/B was detected by ELISA and PCR was used to identify toxin A, toxin B and binary toxin gene in each isolated strain. C. difficile A/B toxins were detected in ten samples (16.7%). Of these, seven were from diarrheic and three were from non-diarrheic piglets. C. difficile was recovered from 12 out of 60... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neonatal diarrhea; Clostridium difficile; Enteritis; Colitis. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000800021 |
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| Silva,Rodrigo Otávio Silveira; Gabardo,Michelle de Paula; Oliveira,Juliana Saes Vilaça de; Lobato,Francisco Carlos Faria; Guedes,Roberto Maurício Carvalho. |
| Lawsonia intracellularisis an obligate intracellular bacterium that is responsible for proliferative enteropathy, an enteric disease endemic in swine and common in foals. However, few studies have investigated this disease in dogs, and there are no reports of dogs infected with L. intracellularisin Latin America. The aim of this study was to evaluate the fecal shedding of L. intracellularisin diarrheic and non-diarrheic dogs in Minas Gerais, Brazil. A total of 58 dogs, 18 apparently healthy and 40 diarrheic, were examined in this study. DNA extracted from feces was analyzed using a nested PCR reaction to detect L. intracellularis.Three out of 40 (7.5%) diarrheic samples, all from 3-month-old puppies, were positive for L. intracellularis. These results... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lawsonia intracellularis; Canine; Proliferative enteropathy; Diarrhea. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000901619 |
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| Silva,Rodrigo Otávio Silveira; Santos,Renata Lara Resende; Pires,Prhiscylla Sadanã; Pereira,Luiz Carlos; Pereira,Silvia Trindade; Duarte,Marina Carvalho; Assis,Ronnie Antunes de; Lobato,Francisco Carlos Faria. |
| The objective of this study was to detect C. difficileA/B toxins and to isolate strains of C. perfringensand C. difficile from diarrheic and non-diarrheic dogs in Brazil. Stool samples were collected from 57 dogs, 35 of which were apparently healthy, and 22 of which were diarrheic. C. difficileA/B toxins were detected by ELISA, and C. perfringensand C. difficilewere identified by multiplex PCR. C. difficileA/B toxins were detected in 21 samples (36.8%). Of these, 16 (76.2%) were from diarrheic dogs, and five (23.8%) were from non-diarrheic dogs. Twelve C. difficile strains (21.1%) were isolated, of which ten were A+B+and two were A-B-. All non-toxigenic strains were isolated from non-diarrheic animals. The binary toxin gene cdtBwas found in one strain,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nosocomial diarrhea; Small animals; Colitis; Enteritis; Canine. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100019 |
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| Silva,Rodrigo Otávio Silveira; Moreira,Felipe Moraghi; Rezende,Jackeline Viana; Pires,Prhiscylla Sadanã; Maranhão,Renata de Pino Albuquerque; Palhares,Maristela Silveira; Lobato,Francisco Carlos Faria. |
| Despite of the substantial role of Clostridium difficile in causing diarrhea and colitis in foals, there have been no confirmed diagnoses of disease caused by this bacteria in Brazil. In this paper, we describe confirmed cases of colitis caused by C. difficile in two foals in Brazil. Two five-month-old foals with a five-day history of diarrhea after antibiotic treatment for a respiratory disease were treated at the Veterinary Hospital of the Universidade Federal de Minas Gerais. C. difficile A/B toxins were detected, and toxigenic strains of C. difficile were isolated from the foals' feces. The treatment was based on fluid therapy and antibiotics (metronidazole and ceftiofur), and the animals experienced a gradual recovery. The association between the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Colitis; Equine; Nosocomial diarrhea. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000300018 |
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| Silva,Rodrigo Otávio Silveira; Ferreira Junior,Francisco Carlos; Marques,Marcus Vinícius Romero; Oliveira Junior,Carlos Augusto; Martins,Nelson Rodrigo da Silva; Lobato,Francisco Carlos Faria. |
| The aim of this study was to isolate, genotype and evaluate the antimicrobial susceptibility of Clostridium perfringens found in species Tinamidae, Cracidae and Ramphastidae in Brazil. C. perfringens was isolated in 13 (5%) out of 260 swab samples and five (8.3%) out of 60 stool samples. All strains were classified as C. perfringens type A, and nine (50%) were positive for the beta-2 toxin-encoding gene. No strains were positive for the necrotic enteritis toxin B-like (NetB)-encoding gene. All isolates were susceptible to penicillin, metronidazole and vancomycin, whereas four (22.2%), five (27.8%) and 13 (72.2%) strains were considered resistant to erythromycin, oxytetracycline and lincomycin, respectively. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Necrotic enteritis; Avian; Toucan. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000300016 |
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| Casagrande,Renata Assis; Pires,Prhiscylla Sadanã; Silva,Rodrigo Otávio Silveira; Sonne,Luciana; Borges,João Batista Souza; Neves,Monique Silva; Rolim,Veronica Machado; Souza,Suyene Oltramari de; Driemeier,David; Lobato,Francisco Carlos Faria. |
| The aim of this study was to report a case of clostridial myocarditis in a bovine in Brazil with emphasis on the pathological findings, isolation and molecular identification associated with the in situ localization of C. chauvoei. The animal, a male Brangus bull with nine months of age, was found dead without prior clinical signs. Multifocal and coalescent areas of necrosis were observed in the myocardium. Rod cells in the cardiac muscle fibers were positive immunostaining for C. chauvoei, while this bacterium was also isolated and identified by polymerase chain reaction (PCR). |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Myonecrosis; Sudden death; Clostridium chauvoei; Myocarditis.. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000801472 |
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| D’Elia,Mirella Lauria; Santos,Alice Barroso; Ribeiro,Beatriz Novaes Telles; Torres,Renato Cesar Sacchetto; Santos,Renato de Lima; Silva,Rodrigo Otávio Silveira; Nepomuceno,Anelise Carvalho. |
| ABSTRACT: A 6-year-old Fuzzy Lop rabbit was referred to a veterinary hospital with a complaint of lameness. In addition to a vertebral subluxation, two radiopaque and well-defined structures were revealed by radiographic evaluation. Ultrasonographically, the masses were characterized as parenchymal structures with diffuse mineralization and formation of reverberation artifacts, suggesting presence of gas. These two structures were excised during a laparotomy and Clostridium tertium was isolated. To the best of our knowledge, this is the first report of C. tertium infection in a pet animal. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clostridia; Granuloma; Diagnostic imaging. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000100551 |
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| Registros recuperados: 31 | |
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